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Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis
https://doi.org/10.50850/0002000064
https://doi.org/10.50850/000200006487c10f58-4fe5-469d-a698-a09df8aeb0ca
| 名前 / ファイル | ライセンス | アクション |
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222DS01博士論文要旨.pdf (354 KB)
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222DS01審査結果要旨.pdf (184 KB)
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222DS01博士論文本文.pdf (3.4 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2025-09-22 | |||||||||
| タイトル | ||||||||||
| タイトル | Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis | |||||||||
| 言語 | en | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | presepsin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | macrophage | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | neutrophil | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | extracellular traps | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | sepsis | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | biomarker | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| 資源タイプ | doctoral thesis | |||||||||
| ID登録 | ||||||||||
| ID登録 | 10.50850/0002000064 | |||||||||
| ID登録タイプ | JaLC | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 著者 |
近藤, 明宏
× 近藤, 明宏
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Presepsin (P-SEP) is a specific biomarker for sepsis. Monocytes produce P-SEP by phagocytosing neutrophil extracellular traps (NETs). Herein, we investigated whether M1 macrophages (M1 MΦs) are the primary producers of P-SEP after NET phagocytosis. We co-cultured M1 MΦs and NETs from healthy participants, measured P-SEP levels in the culture medium supernatant, and detected P-SEP using western blotting. When NETs were co-cultured with M1 MΦs, the P-SEP level of the culture supernatant was high. Notably, we demonstrated, for the first time, the intracellular kinetics of P-SEP production by M1 MΦs via NET phagocytosis: M1 MΦs produced P-SEP intracellularly 15 min after NET phagocytosis and then released it extracellularly. In a sepsis mouse model, the blood NET ratio and P-SEP levels, detected using ELISA, were significantly increased (p < 0.0001). Intracellular P-SEP analysis via flow cytometry demonstrated that lung, liver, and kidney MΦs produced large amounts of P-SEP. Therefore, we identified these organs as the origin of M1 MΦs that produce P-SEP during sepsis. Our data indicate that the P-SEP level reflects the trend of NETs, suggesting that monitoring P-SEP can be used to both assess NET-induced organ damage in the lungs, liver, and kidneys during sepsis and determine treatment efficacy. | |||||||||
| 言語 | en | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士(臨床検査学) | |||||||||
| item_10006_degree_grantor_9 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 26201 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 香川県立保健医療大学 | |||||||||
| 学位授与年度 | ||||||||||
| ja | ||||||||||
| 2024 | ||||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2025-03-09 | |||||||||
| dissertation_number | ||||||||||
| 学位授与番号 | 甲第8号 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | AM | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
| bibliographic_information |
en : Scientific Reports 巻 14, 号 1, p. 16386-16386, 発行日 2024-07-16 |
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| item_10006_relation_31 | ||||||||||
| 識別子タイプ | DOI | |||||||||
