{"created":"2025-09-22T02:44:16.505090+00:00","id":2000064,"links":{},"metadata":{"_buckets":{"deposit":"44647428-b1ab-4267-80fe-6e7ea12efc98"},"_deposit":{"created_by":5,"id":"2000064","owner":"5","owners":[5],"pid":{"revision_id":0,"type":"depid","value":"2000064"},"status":"published"},"_oai":{"id":"oai:kagawa-puhs.repo.nii.ac.jp:02000064","sets":["38:101"]},"author_link":[],"control_number":"2000064","item_10006_biblio_info_27":{"attribute_name":"bibliographic_information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-07-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"16386","bibliographicPageStart":"16386","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"Scientific Reports","bibliographic_titleLang":"en"}]}]},"item_10006_date_granted_11":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2025-03-09"}]},"item_10006_degree_grantor_9":{"attribute_name":"item_10006_degree_grantor_9","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_language":"ja","subitem_degreegrantor_name":"香川県立保健医療大学"}],"subitem_degreegrantor_identifier":[{"subitem_degreegrantor_identifier_name":"26201","subitem_degreegrantor_identifier_scheme":"kakenhi"}]}]},"item_10006_degree_name_8":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(臨床検査学)","subitem_degreename_language":"ja"}]},"item_10006_description_7":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Presepsin (P-SEP) is a specific biomarker for sepsis. Monocytes produce P-SEP by phagocytosing neutrophil extracellular traps (NETs). Herein, we investigated whether M1 macrophages (M1 MΦs) are the primary producers of P-SEP after NET phagocytosis. We co-cultured M1 MΦs and NETs from healthy participants, measured P-SEP levels in the culture medium supernatant, and detected P-SEP using western blotting. When NETs were co-cultured with M1 MΦs, the P-SEP level of the culture supernatant was high. Notably, we demonstrated, for the first time, the intracellular kinetics of P-SEP production by M1 MΦs via NET phagocytosis: M1 MΦs produced P-SEP intracellularly 15 min after NET phagocytosis and then released it extracellularly. In a sepsis mouse model, the blood NET ratio and P-SEP levels, detected using ELISA, were significantly increased (p < 0.0001). Intracellular P-SEP analysis via flow cytometry demonstrated that lung, liver, and kidney MΦs produced large amounts of P-SEP. Therefore, we identified these organs as the origin of M1 MΦs that produce P-SEP during sepsis. Our data indicate that the P-SEP level reflects the trend of NETs, suggesting that monitoring P-SEP can be used to both assess NET-induced organ damage in the lungs, liver, and kidneys during sepsis and determine treatment efficacy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10006_dissertation_number_12":{"attribute_name":"dissertation_number","attribute_value_mlt":[{"subitem_dissertationnumber":"甲第8号"}]},"item_10006_identifier_registration":{"attribute_name":"identifier_registration","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.50850/0002000064","subitem_identifier_reg_type":"JaLC"}]},"item_10006_relation_31":{"attribute_name":"item_10006_relation_31","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_select":"DOI"}}]},"item_10006_text_10":{"attribute_name":"学位授与年度","attribute_value_mlt":[{"subitem_text_language":"ja","subitem_text_value":"2024"}]},"item_10006_version_type_18":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kondo, Akihiro","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-09-22"}],"filename":"222DS01博士論文要旨.pdf","filesize":[{"value":"354 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kagawa-puhs.repo.nii.ac.jp/record/2000064/files/222DS01博士論文要旨.pdf"},"version_id":"52371634-6fc6-45cf-93b8-58c8d2efdb95"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2025-09-22"}],"filename":"222DS01審査結果要旨.pdf","filesize":[{"value":"184 KB"}],"format":"application/pdf","mimetype":"application/pdf","url":{"url":"https://kagawa-puhs.repo.nii.ac.jp/record/2000064/files/222DS01審査結果要旨.pdf"},"version_id":"dd996efb-53dc-407b-b11d-e208f3096c2d"},{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2026-01-22"}],"filename":"222DS01博士論文本文.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensetype":"license_0","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://kagawa-puhs.repo.nii.ac.jp/record/2000064/files/222DS01博士論文本文.pdf"},"version_id":"de8d2668-d554-4daa-a34a-952ff0603657"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"presepsin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"macrophage","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"neutrophil","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular traps","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sepsis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"biomarker","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"item_resource_type","attribute_value_mlt":[{"resourcetype":"doctoral thesis","resourceuri":"http://purl.org/coar/resource_type/c_db06"}]},"item_title":"Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis","subitem_title_language":"en"}]},"item_type_id":"10006","owner":"5","path":["101"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2025-09-22"},"publish_date":"2025-09-22","publish_status":"0","recid":"2000064","relation_version_is_last":true,"title":["Identification of organs of origin of macrophages that produce presepsin via neutrophil extracellulart trap phagocytosis"],"weko_creator_id":"5","weko_shared_id":-1},"updated":"2026-01-29T11:10:53.334433+00:00"}